Ultraviolet B (UVB) radiation is the main cause of photoaging processes including cellular senescence, skin drying, collagen\ndegradation, melanogenesis, and inflammation. these responses occur because UVB induces a change in expression of agingrelated\ngenes through regulation of signal pathways such as that of mitogen-activated protein kinases- (MAPKs-) activator protein\n1 (AP-1). Ranunculus bulumei, which is used as an herb in Indonesia, belongs to the Ranunculaceae family, which has been\nreported to perform various physiological effects including antioxidant and anti-inflammation. However, data on the pharmaceutical\nand cosmeceutical utility of Ranunculus bulumei have not been reported. Therefore, we evaluated the antiaging efficacy\nof RB-ME, a methanol extract of Ranunculus bulumei. Rb-ME attenuated MMP9 and COX-2 gene expression but enhanced SIRT1\nand type-1 collagen in UVB-irradiated HaCaT cells. Rb-ME regulated these gene expressions through inhibition of p38\nphosphorylation and inactivation of AP-1. In addition, mRNA expression of HAS-2 and -3, which are involved in skin hydration,\nwas elevated in Rb-ME-treated HaCaTcells. Rb-ME also inhibited melanogenesis by suppression of tyrosinase, MITF, and TYRP-\n1 mRNA in B16F10 cells under Alpha-MSH treatment. Taken together, these results indicate that Rb-ME has a protective effect on\nsome UVB-induced skin photoaging events such as inflammation, collagen degradation, cellular senescence, skin drying, and\nmelanin production through inhibition of the p38-AP-1 signal cascade, indicating that Rb-ME can be used as an active ingredient\nfor antiaging cosmetics.
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